• Bio-One Inc Slider-1
  • Bio-One Inc Slider-2
  • Bio-One Inc Slider-3
  • Bio-One Inc Slider-4
  • Bio-One Inc Slider-5

Bio-One Inc - Franchise


Quick Summary

Bio-One Inc. leads the field in biohazard and decontamination cleanup services, with over 100 franchise owners operating 130+ locations across 45 states. Responding to people's most critical needs with discretion and compassion, Bio-One is dedicated to helping communities in distress. Our mission is simple: Help First, Business Second. As community heroes, our franchisees tackle tasks others won't, from biohazard cleanup to hoarding and mold remediation, with professionalism and care. Acquired by Five Star Franchising in 2021, Bio-One is primed for rapid expansion, offering low start-up costs and high-profit margins in this recession-resilient industry. Join us and make a difference in your community.

*Please contact "Bio-One Inc" to confirm the accuracy of any information provided on this page.

Bio-One Inc
Business Cost, Fees & Facts for 2025

Minimum Cash Required $50,000
Year Founded 2006
# of Existing Units 140
Training & Support Yes
Military/Veteran Promotion Yes
View More Quick Facts

Available In These States:

We are currently accepting inquiries in these states:
  • AK
  • AR
  • AZ
  • CA
  • CO
  • CT
  • DC
  • DE
  • FL
  • GA
  • IA
  • ID
  • IL
  • IN
  • KS
  • KY
  • LA
  • MA
  • MD
  • ME
  • MI
  • MN
  • MO
  • MS
  • MT
  • NC
  • ND
  • NH
  • NJ
  • NM
  • NY
  • OH
  • OR
  • PA
  • RI
  • SC
  • SD
  • TN
  • TX
  • UT
  • VA
  • VT
  • WA
  • WI
  • WV
  • WY
  • Overview
  • Military & Veteran
  • Testimonials
  • Video

Bio-One Inc - Franchise

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion.

Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities.

Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

Why Bio-One?
As a Franchise Owner, you'll support and care for others during life's most difficult moments. Serve your community by providing a wide range of cleanup services that also create multiple revenue streams for your business. You don’t need any previous experience to own a Bio-One franchise. Just bring your heart for service and we’ll provide you with all the tools and training you need to run your business.

  • Recession-Resilient: Our services are consistently in demand, regardless of economic factors.
  • Non-Seasonal: Bio-One operates 24/7/365, ensuring continuous business regardless of the season.
  • Limited Competition: In a growing industry with few competitors, your business stands out locally.
  • Limited Staff Needed: Few technicians are required for each job, streamlining operations.
  • Category-Leading Brand: Bio-One has empowered owner success since 2010 as the industry's largest and first-to-franchise in this niche.
  • High Tickets & Strong Margins: We specialize in high-value insurance-based jobs with attractive margins.
  • Fast Start-Up: We offer comprehensive training and support, ensuring a swift and efficient opening and ongoing operation.
  • Low Start-Up Costs: Our business model features low initial investment and scalability, with no need for expensive equipment.
  • Manageable Labor: Start with a few highly trained technicians to kickstart your business.
  • Five Star Support: Benefit from specialized training, expertise, partnerships, and ongoing support to maximize your growth potential.

Bio-One Services
Bio-One thrives by following a basic premise: provide top-notch decontamination and biohazard cleanup services with discretion, care, and compassion. Bio-One Answers the call with extensive services for the most challenging and sensitive jobs.

  • Crime Scene Cleanup
  • Decomposition / Undiscovered Death
  • Meth Lab / Fentanyl Cleanup
  • Virus / Bacteria Disinfection
  • Odor Removal
  • Homeless Encampment Cleanup
  • Blood Spill Cleanup
  • Feces / Urine Cleanup
  • Biorecovery Mold Remediation
  • Emergency Vehicle Decontamination
  • Teargas Cleanup
  • Hoarding Cleanup
  • Gross Filth Cleaning
  • Animal Hoarding Cleanup
  • Rodent Droppings
  • Medical Waste

Why This Industry
Let’s face it. Burnout and stress are on the rise adding to the growing mental health crisis. Violent crimes happen everywhere and in some cases and places, are happening more frequently. Contributing factors like inflation and economic uncertainty contribute to these trends. Bio-One exists to answer the call for reliable, trusted, and compassion services communities everywhere require now more than ever.

  • Recession-Resilient - The services we provide will always be needed regardless of macro-economic factors.
  • Not Seasonal - This business happens 24 / 7 / 365 regardless of season.
  • Limited Competition - Growing industry with few businesses in this niche helping you stand out locally.
  • Limited Staff Needed - Very few technicians needed on a job.

Key Differentiators:

  • First to Franchise Category Leader
  • Niche specialized services in demand
  • Purpose-Driven Team and Owner Network
  • Comprehensive Training, Support and Coaching
  • Backed by Five Star Franchising and Princeton Equity

Top 5 Selling Points:

  • Fast Startup with Low Initial Investment
  • Home-based and Low Overhead
  • Multiple Service Lines = Multiple Revenue Streams
  • No Experience Needed
  • Robust Training and Support

Veteran Discount - $9,000 Off Initial Franchise Fee (15% off); First Responder Discount - $9,000 Off Initial Franchise Fee (15% off)

“What's been rewarding the most is just that we were able to do it. We were able to take this challenge on and conquer it and far exceed our goals."John, Bio-One of Charlotte, NC
“It's huge to have that support with someone that's been there, someone that knows what's going on.”Angela, Bio-One of Madison, WI
“Bio-One was a great fit where you're both serving the social good, but you can build a business, you can support your community by creating opportunities.”Matt, Bio-One of Asheville, NC and Denver, CO
“The Bio-One support system is incredible. They are a phone call away, a text away, an email away. We have found that anything we need whether it’s about how to bid a job, how to work with an insurance company or client, they are there and they have a wonderful program to teach us how to make our business successful. We originally looked at buying different businesses. With Bio-One, we couldn’t imagine doing anything different.”Vicky and Darren, Bio-One Western Slope

Here are some other suggestions in the same category that you selected :

HHO Carbon Clean Systems

** Join this first-mover to offer fuel savings and improved engine performance ** With little to no competition in U.S. markets, HHO is a first-to-market opportunity, no diesel or automotive background required. Being a fully mobile preventative service business means a low overhead expense for the owner. The opportunity is ideal for visionaries looking for a unique business venture that benefits the community by lowering emissions and costly downtime, ultimately saving the customers money.

Read More »

$ 100,000 Min.Cash Required

Fibrenew

Fibrenew is the world's largest franchise in leather, plastic & vinyl Restoration. With 300+ Franchise Partners in 6 countries, 35+ years of history, and plenty of room for expansion, now is the time to look at the Fibrenew opportunity.

Read More »

$ 25,000 Min.Cash Required

FranDestiny

Have you dreamed of owning your own franchise business but don’t know where to start? FranDestiny provides a consultation service to prospective franchise business owners that results in matching the best franchises with our candidates. Our process can save you time and money in your search, provide you with intelligent choices based on your goals and preferences and, best of all, it’s FREE TO YOU. Your fees are the same with or without our guidance, so you have nothing to lose. Learn more now!

Read More »

$ 150,000 Min.Cash Required

PuroClean

Every day, there are more than 50,000 water damage property losses in the United States. PuroClean is in search of business professionals who want to become a superhero in their local community and help make a difference in people's lives.

Read More »

$ 100,000 Min.Cash Required

TruBlue Home Service Ally

TruBlue is working to ease the burden of home upkeep for busy families and seniors aging-in-place through senior modifications, home maintenance, handyman services, and ongoing maintenance programs. Our franchisees have the opportunity to run a successful franchising business while making a difference in their communities. Learn more today!

Read More »

$ 50,000 Min.Cash Required

Restoration 1

Restoration1 is a unique opportunity providing a wide array of emergency mitigation, restoration, and reconstruction services. These services are always in high demand and are a necessity, so they are never affected by the economy. Learn more today about this top restoration franchise!

Read More »

$ 50,000 Min.Cash Required

The Entrepreneur's Source - Navigate Your Path to Business Ownership!

The Entrepreneur's Source is a premier Career Ownership Coaching® business dedicated to helping individuals explore franchising opportunities & achieve self-sufficiency. We specialize in navigating the complexities of franchising, guiding aspiring entrepreneurs through a proven process to uncover their goals & needs. With personalized coaching & comprehensive resources, we empower you to find the right franchise that aligns with your lifestyle—at no cost to you. Discover your path with us today!

Read More »

$ 50,000 Min.Cash Required

Fresh Coat

Fresh Coat is North America’s leading high-volume interior painting company. Be the boss - don’t paint! Build an organization in an industry with high income potential. Fresh Coat is a turn-key franchise offering high profit margins and low start-up costs. Inquire now to learn more.

Read More »

$ 50,000 Min.Cash Required

Dryer Vent Wizard

Dryer Vent Wizard, a Neighborly company, is the nation's leading dryer vent cleaning and repair services franchise that capitalizes on a niche industry.

Read More »

$ 30,000 Min.Cash Required

Fleet Services International

Exclusive territory. #1 Industry leader since 2001. 100% financing for U.S. citizens who qualify* plus software package with complete training. $100K 1st year profit potential. National alliances...

Read More »

$ 100,000 Min.Cash Required

Junk King

Junk King, a Neighborly company, is the nation's leading junk removal franchise. With us, your path to business ownership can experience the royal treatment!

Read More »

$ 50,000 Min.Cash Required

Mr. Handyman

Mr. Handyman, a Neighborly company, is the nation's leading home improvement services franchise. With us, you can build your ideal business!

Read More »

$ 50,000 Min.Cash Required

Rytech Water Damage & Mold Specialists

If you are searching for a rewarding and profitable business to own, look no further than Rytech. We are an established service provider franchise in a virtually recession-proof industry. Water and fire restoration along with mold remediation services are part of a multibillion-dollar industry that offers potential franchisees the opportunity to build a successful business. Learn more about becoming part of this ever-growing industry today and reach out to our franchising team.

Read More »

$ 75,000 Min.Cash Required

The Brothers That Just Do Gutters

Invest in a first-to-market, national brand with an average franchisee gross sales of $1.7M! This family-built brand has huge momentum, with 400+ franchise locations, incredible customer validation, and over 22 years of perfecting the business.

Read More »

$ 80,000 Min.Cash Required

Poolwerx

Backed by a global brand, Poolwerx partners can tap into the $7 billion swimming pool industry with multiple revenue streams including chemical, equipment, and retail sales plus year-round revenue from residential and commercial pool services like cleaning, maintenance, and repairs. Scale your business with us as we rapidly expand our footprint across the US!

Read More »

$ 80,000 Min.Cash Required

Purchase A Franchise Using Your Retirement Plan

This is NOT a franchise or business opportunity. This is a service offered to those seeking to purchase a franchise or business using their retirement funds without having to pay the taxes and penalties. You can purchase a franchise or traditional business with a tax qualified retirement plan such as an IRA, 401(k), 403(b), 457(b), SEPs and others.

Read More »

$ 30,000 Min.Cash Required

Kitchen Tune-Up

Kitchen Tune-Up® has been updating, upgrading, and uplifting kitchens for over 35 years. Our outstanding remodeling concept offers personalized service and kitchen updates that are structured around our customer service Trustpoints to ensure homeowners get an upgraded experience from start to finish. Our franchise opportunity appeals to entrepreneurs transitioning into business ownership from corporate backgrounds. Learn more today!

Read More »

$ 80,000 Min.Cash Required

Aire Serv

Aire Serv, a Neighborly company, is the nation's leading heating, ventilation, and air conditioning services franchise. With us, you'll stay cool while your business heats up!

Read More »

$ 50,000 Min.Cash Required

Budget Blinds

Budget Blinds® is the #1 window coverings franchise in North America. Our franchisees offer convenient shop-at-home service for window coverings including blinds, shades, shutters, and drapes. We help you succeed with our low inventory, low overhead, home-based business model and ongoing support. Learn more now!

Read More »

$ 80,000 Min.Cash Required

Mr. Appliance

Mr. Appliance, a Neighborly company, is the nation's leading appliance repair services franchise.

Read More »

$ 85,000 Min.Cash Required

Footprints Floors

Join one of the fastest-growing and most simple-to-run home-service brands. No build-out, no inventory, and no employees required. All marketing and customer acquisition is completed for you by the corporate team!

Read More »

$ 75,000 Min.Cash Required

Add Businesses and/or Franchises
To Basket For Free Information

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies. Review our cookies information for more details.