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Bio-One Inc - Franchise


Quick Summary

Bio-One Inc. leads the field in biohazard and decontamination cleanup services, with over 100 franchise owners operating 130+ locations across 45 states. Responding to people's most critical needs with discretion and compassion, Bio-One is dedicated to helping communities in distress. Our mission is simple: Help First, Business Second. As community heroes, our franchisees tackle tasks others won't, from biohazard cleanup to hoarding and mold remediation, with professionalism and care. Acquired by Five Star Franchising in 2021, Bio-One is primed for rapid expansion, offering low start-up costs and high-profit margins in this recession-resilient industry. Join us and make a difference in your community.

*Please contact "Bio-One Inc" to confirm the accuracy of any information provided on this page.

Bio-One Inc
Business Cost, Fees & Facts for 2025

Minimum Cash Required $50,000
Year Founded 2006
# of Existing Units 140
Training & Support Yes
Military/Veteran Promotion Yes
View More Quick Facts

Available In These States:

We are currently accepting inquiries in these states:
    • Overview
    • Military & Veteran
    • Testimonials
    • Video

    Bio-One Inc - Franchise

    Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion.

    Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities.

    Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

    Why Bio-One?
    As a Franchise Owner, you'll support and care for others during life's most difficult moments. Serve your community by providing a wide range of cleanup services that also create multiple revenue streams for your business. You don’t need any previous experience to own a Bio-One franchise. Just bring your heart for service and we’ll provide you with all the tools and training you need to run your business.

    • Recession-Resilient: Our services are consistently in demand, regardless of economic factors.
    • Non-Seasonal: Bio-One operates 24/7/365, ensuring continuous business regardless of the season.
    • Limited Competition: In a growing industry with few competitors, your business stands out locally.
    • Limited Staff Needed: Few technicians are required for each job, streamlining operations.
    • Category-Leading Brand: Bio-One has empowered owner success since 2010 as the industry's largest and first-to-franchise in this niche.
    • High Tickets & Strong Margins: We specialize in high-value insurance-based jobs with attractive margins.
    • Fast Start-Up: We offer comprehensive training and support, ensuring a swift and efficient opening and ongoing operation.
    • Low Start-Up Costs: Our business model features low initial investment and scalability, with no need for expensive equipment.
    • Manageable Labor: Start with a few highly trained technicians to kickstart your business.
    • Five Star Support: Benefit from specialized training, expertise, partnerships, and ongoing support to maximize your growth potential.

    Bio-One Services
    Bio-One thrives by following a basic premise: provide top-notch decontamination and biohazard cleanup services with discretion, care, and compassion. Bio-One Answers the call with extensive services for the most challenging and sensitive jobs.

    • Crime Scene Cleanup
    • Decomposition / Undiscovered Death
    • Meth Lab / Fentanyl Cleanup
    • Virus / Bacteria Disinfection
    • Odor Removal
    • Homeless Encampment Cleanup
    • Blood Spill Cleanup
    • Feces / Urine Cleanup
    • Biorecovery Mold Remediation
    • Emergency Vehicle Decontamination
    • Teargas Cleanup
    • Hoarding Cleanup
    • Gross Filth Cleaning
    • Animal Hoarding Cleanup
    • Rodent Droppings
    • Medical Waste

    Why This Industry
    Let’s face it. Burnout and stress are on the rise adding to the growing mental health crisis. Violent crimes happen everywhere and in some cases and places, are happening more frequently. Contributing factors like inflation and economic uncertainty contribute to these trends. Bio-One exists to answer the call for reliable, trusted, and compassion services communities everywhere require now more than ever.

    • Recession-Resilient - The services we provide will always be needed regardless of macro-economic factors.
    • Not Seasonal - This business happens 24 / 7 / 365 regardless of season.
    • Limited Competition - Growing industry with few businesses in this niche helping you stand out locally.
    • Limited Staff Needed - Very few technicians needed on a job.

    Key Differentiators:

    • First to Franchise Category Leader
    • Niche specialized services in demand
    • Purpose-Driven Team and Owner Network
    • Comprehensive Training, Support and Coaching
    • Backed by Five Star Franchising and Princeton Equity

    Top 5 Selling Points:

    • Fast Startup with Low Initial Investment
    • Home-based and Low Overhead
    • Multiple Service Lines = Multiple Revenue Streams
    • No Experience Needed
    • Robust Training and Support

    Veteran Discount - $9,000 Off Initial Franchise Fee (15% off); First Responder Discount - $9,000 Off Initial Franchise Fee (15% off)

    “What's been rewarding the most is just that we were able to do it. We were able to take this challenge on and conquer it and far exceed our goals."John, Bio-One of Charlotte, NC
    “It's huge to have that support with someone that's been there, someone that knows what's going on.”Angela, Bio-One of Madison, WI
    “Bio-One was a great fit where you're both serving the social good, but you can build a business, you can support your community by creating opportunities.”Matt, Bio-One of Asheville, NC and Denver, CO
    “The Bio-One support system is incredible. They are a phone call away, a text away, an email away. We have found that anything we need whether it’s about how to bid a job, how to work with an insurance company or client, they are there and they have a wonderful program to teach us how to make our business successful. We originally looked at buying different businesses. With Bio-One, we couldn’t imagine doing anything different.”Vicky and Darren, Bio-One Western Slope

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